IN VITRO PROPAGATION OF ORIGANUM SYRIACUM AND ORIGANUM EHRENBERGII
Many oregano species are growing wild in the Mediterranean basin where they are often submitted to overharvest practices that are seriously threatening the sustainability of these resources. Cultivation of these species is highly recommended to reduce the overexploitation of the spontaneous forms. Difficulties in attempting conventional propagation have been reported due to the poor germinability of the seeds and the serious rooting problems associated with cuttings. The present study aimed to investigate the potential of in vitro propagation for Origanum syriacum L. (endemic to the Levantine) and O. ehrenbergii (endemic to Lebanon). Multiple shoots were obtained from nodal segments on MS medium supplemented with different concentrations of BAP (1, 1.5, 2 mg/L). Shoot multiplication was conducted on the same media by subculturing nodal segments with 2-3 nodes every four weeks. After four subcultures, multiplication was achieved with a mean rate of 2.0 to 3.7 new shootlets per explant. High rooting response of shoots (100%) was obtained on MS basal medium deprived of growth regulators. Globally, the species O. ehrenbergii has proved to be more reactive than O. syriacum during the different stages of cultures initiation, multiplication and rooting. These results indicate the important potential of in vitro propagation to be used for large scale multiplication of oregano allowing thereby its cultivation and contributing to the sustainable use of biological diversity.
El Beyrouthy, M., Elian, G., Abou Jaoudeh, C. and Chalak, L. (2015). IN VITRO PROPAGATION OF ORIGANUM SYRIACUM AND ORIGANUM EHRENBERGII. Acta Hortic. 1083, 169-172
micropropagation, oregano, species, tissue culture