CALLUS-MEDIATED PLANT REGENERATION FROM VARIEGATED LEAVES PRODUCED BY MUTAGEN-TREATED TOMATO MERISTEM LEAF PRIMORDIA
Meristematic tissues removed from aseptically cultivated tomato seedlings (Solanum lycopersicum Mill.), breeding line KT007, were treated with two chemical mutagens (N-methyl-N-nitro-N-nitrosoguanidine and 5-azacytosine), and growth and differentiation or morphological changes in the mutagen-treated leaf primordia were traced in developed leaves. Morphological variations were most frequently detected in leaves from meristems treated with 100 μg/ml N-methyl-N-nitro- N-nitrosoguanidine. Variegated leaves were cultured to obtain callus tissues for in vitro isolation and to propagate morphologically altered tomato plants using Murashige-Skoog medium supplemented with different concentrations of 6-benzylaminopurine and 2,4-dichlorophenoxyacetic acid. Eventually, calli were obtained from the leaves and successfully differentiated into plants with variegated leaves using medium supplemented with 2.0 μg/ml 6-benzylaminopurine. Consequently, the regenerated plants produced variegated leaves similar to those in the plants originally used for tissue culture. This approach using chemical mutagens is an easy and effective way to induce variegated leaves and to rapidly propagate their regenerants in vitro. Our results demonstrate that this approach is an effective method for easily and rapidly inducing variations in tomato leaves and for analysing tomato leaf morphogenesis through regeneration based on an in vitro tissue culture system.
Matsuda, Y., Nonomura, T. and Toyoda, H. (2015). CALLUS-MEDIATED PLANT REGENERATION FROM VARIEGATED LEAVES PRODUCED BY MUTAGEN-TREATED TOMATO MERISTEM LEAF PRIMORDIA . Acta Hortic. 1083, 475-480
leaf primordium, mutagens, plant regeneration