PHOTOMORPHOGENESIS BY LED LIGHTING ON POTATO AND BRAZILIAN GINSENG FOR MEDIUM-TERM IN VITRO CONSERVATION
Light emitting diodes (LEDs) are being substituted for fluorescent light on some plant in vitro cultures due to the lower energy consumption and longer durability. The effects of LED lighting on the in vitro growth of nodal segments of potato (Solanum tuberosum) and Brazilian ginseng (Pfaffia glomerata) germplasms were studied for medium-term in vitro conservation. Nodal segments of three accessions of potato and Brazilian ginseng were cultured on the MS medium without growth regulator at 20°C under illuminations by blue LEDs (peak wavelength: 450 nm), red LEDs (peak wavelength: 650 nm) and white LEDs (color temperature: 6400 K) at photosynthetic photon flux density of 30 µmol m-2 s-1 with a 12-h photoperiod. LED lighting effect on in vitro plantlets varied depending on in vitro culture period and plant species. The blue and red LEDs showed similar growth efficiency on in-vitro plantlets compared with the white LEDs. However, there are trends of reductions in plantlet height under the blue LEDs and root length under red LEDs. The senescent leaf frequency of the potato plantlets under the blue LEDs was reduced in comparison with the white and red LEDs. It is suggested that the blue LEDs are possible to use for the slow growth in vitro conservation of germplasms.
Luz, T.C.L.A., Cardoso, L.D., Alves, R.B.N. and Matsumoto, K. (2015). PHOTOMORPHOGENESIS BY LED LIGHTING ON POTATO AND BRAZILIAN GINSENG FOR MEDIUM-TERM IN VITRO CONSERVATION . Acta Hortic. 1083, 513-517
blue LED, micropropagation, Pfaffia glomerata, red LED, slow growth, Solanum tuberosum