CHEMICAL AND PHYSICAL CHANGES IN THE ENVIRONMENT OF PINUS RADIATE EMBRYOGENIC TISSUE ALONG INITIATION STAGE: IMPLICATIONS IN THE SOMATIC PLANTLET DEVELOPMENT
Drought and increased temperature are the most remarkable effects that climate change will have over plant growth and distribution. In this sense, epigenetic variation seems to contribute to the phenotypic plasticity and adaptative capacity of plants. In the last years our research team has worked optimising the in vitro micropropagation of Pinus radiate via somatic embryogenesis. This fact gives us a potent biotechnological tool to manipulate the environmental conditions during the micropropagation process, and enables us to obtain a great amount of clonal material and then, to carry out studies to analyse how plantlets behave in their ex vitro development under different conditions and what level of stress tolerance they have acquired. The overall aim of our study is to be able to modulate the characteristics of the plantlets produced by manipulating the environmental conditions during the somatic embryogenesis process; this is a long-term objective as after growing the somatic plantlets we will analyse the effect of different stresses in these plants. As a short term objective, we want to study if it is possible to initiate and to proliferate embryogenic tissue under stressful conditions of temperature and water availability, and evaluate if embryogenic tissue initiation is a critical stage to modulate the quality of the plantlets produced at the end of the embryogenic process. We have found that the embryogenic tissue initiated under different temperatures performed differently after proliferation, thus these embryogenic tissues seem to have a memory to remember the conditions in which they have been initiated. We have also been capable of regenerating somatic embryos from the embryogenic cell lines initiated under stressful conditions.
García-Mendiguren, O., Goicoa, T., Ugarte, M.D., Montalbán, I.A. and Moncaleán, P. (2015). CHEMICAL AND PHYSICAL CHANGES IN THE ENVIRONMENT OF PINUS RADIATE EMBRYOGENIC TISSUE ALONG INITIATION STAGE: IMPLICATIONS IN THE SOMATIC PLANTLET DEVELOPMENT. Acta Hortic. 1083, 95-102
conifer, micropropagation, radiata pine, somatic embryogenesis, tissue culture