ROLE OF PROTECTIVE ENZYMES IN TOMATO ROOTSTOCKS TO RESIST ROOT KNOT NEMATODES

L.K. Yin, W.C. Zhao, C. Shu, X.M. Li, J.W. Fan , S.H. Wang
In recent years, root knot nematodes (RKN) are causing massive produce losses in vegetables. Grafting can effectively reduce the root knot nematode disease if proper stocks resistant to this disease are used. In this study, 11 tomato stocks from home and abroad were used to measure four kinds of protective enzymes including phenylalanine ammonia enzyme (PAL), polyphenol oxidase (PPO), peroxidase (POD) and superoxide dismutase (SOD), to differentiate the role of the protective enzymes play in the resistance to RKN. Results shows that ‘LA0656’ has the highest SOD level among all materials at 0.605 U/g FW, and broadly, the cultivars which have Mi gene have higher SOD activities and those without Mi gene have lower SOD activities. ‘LA3601’ have the highest levels of PPO activity, reaching 0.761 ΔOD/(g·min) FW, followed by ‘CH101’ and ‘LA0656’, while ‘LA2822’ and ‘LA2826’ nearly get a 0. ‘LA2822’ have highest PAL levels among all the cultivars, reaching 5.444 U/g FW, and there are some cultivars which get extremely low PAL levels, such as ‘LA0655’, ‘LA0656’, ‘LA3858’ and ‘HA4402’. POD enzyme levels of ‘LA2822’, ‘LA3640’ are almost 0, while the highest reached over 50ΔOD/(g·min) FW in ‘LA0656’ and ‘LA2157’. Our results indicate a complex relationship between enzyme activities and resistance, to help enclose what role the protective enzymes play in resistance mechanism of different tomato cultivars, choose better stocks and it also provide theoretical basis for further breeding work.
Yin, L.K., Zhao, W.C., Shu, C., Li, X.M., Fan , J.W. and Wang, S.H. (2015). ROLE OF PROTECTIVE ENZYMES IN TOMATO ROOTSTOCKS TO RESIST ROOT KNOT NEMATODES. Acta Hortic. 1086, 213-218
DOI: 10.17660/ActaHortic.2015.1086.26
https://doi.org/10.17660/ActaHortic.2015.1086.26
tomato, root knot nematode, enzyme activity, graft, rootstock
English

Acta Horticulturae