PETUNIA ×HYBRID (HOOK.) VILM. WITH BICOLOR FLOWERS, OBTAINED BY CHEMICAL MUTAGENESIS
To achieve variability in flower coloration of ornamental plants breeders use different approaches. However, bicolor cultivars, bearing flowers with different dyeing centers and edges, are rare. In our work, plants with bicolor corollas were obtained by chemical mutagenesis. Seeds of petunia cultivar Snow ball with white flowers were treated with solutions of ethylmethansulphonat (EMS) for 16 h. In order to obtain М2-М4 progeny the first flowers were isolated and artificially self-pollinated before bud dehiscence. The seeds were collected from individual plants. The flavonoids from wild type plants and selected mutant lines were extracted with acid alcohol from fresh limbs. For detecting anthocyanins and flavonols we used HPLC analysis. We obtained mutant lines with violet-blue limbs with purple rose center (no. 33-9-7) and purple-blue limbs with lavender-rose center (33-5-1). The lobes and lobes fusion places have blue color in both cases. Biochemical analysis showed that mutant plants corollas contain 15 to 20 different anthocyanins with various aglycones such as delphinidin, petunidin, malvidin and cyanidin. The line 33-9-7 contains delphinidin, petunidin and malvidin glycosides in the rose and blue part (35 and 70 mg/L, respectively), and corollas of line 33-5-1 contain the same compounds (11 and 23 mg/L) and even cyanidin. In the line 33-5-1 the corolla lobes have higher amount of delphinidin while methylated anthocyanins are found mainly in the flower center. However, flavonols as kaempferol and quercetin glycosides were found in large amounts (590-1100 mg/L) in mutant flowers. The flavonols concentration was 35-50 times higher than of anthocyanins in the rose part of the corolla. Thus, mutagens lead to changes in the flavonoid biosynthesis in the different parts of petunia corollas.
Shirokova, A.V., Zaitsev, G., Nikolayeva, T.N., Zagoskina, N., Kostyanovskiy, R., Krutius, O., Kadorkina, G. and Semenov, N.N. (2015). PETUNIA ×HYBRID (HOOK.) VILM. WITH BICOLOR FLOWERS, OBTAINED BY CHEMICAL MUTAGENESIS. Acta Hortic. 1087, 141-146
ornamental plants, anthocyanins, ethylmethansulphonat, flower color