INTERSPECIFIC HYBRIDIZATION IN THE TRIBE GENISTEAE USING IN VITRO EMBRYO RESCUE
Since 2005, the Eurogeni GIE , in cooperation with joint research unit IRHS (Institut de Recherche en Horticulture et Semences), has developed a breeding program within the tribe Genisteae (Fabaceae) using interspecific hybridization with the aim to obtain innovative hybrids with novel flower colors. To overcome post-zygotic incompatibility and embryo abortion in vitro embryo rescue was performed. In total 13 interspecific cross combinations between Cytisus scoparius, Cytisus supinus and Cytisus × boskoopii as female parents and Cytisus purpureus as male parent were performed. Immature fruits were collected 4 to 6 days after pollination and ovules were initiated in vitro. Germination of the ovules occurred 1 to 9 months later. In total, 38 F1 seedlings were obtained, with at least one seedling for each cross combination. Only 29 F1 seedlings survived until rooting in vitro and 22 F1 seedlings are already acclimatized in the greenhouse. Those seedlings were determined as hybrids through molecular ISSR markers and flow cytometric measurements. Based on these genome size determinations, 3 hybrids appeared to be tetraploid (4.3 pg/2C), while all parent plants are diploid (1.9 pg/2C - 2.4 pg/2C). The available hybrids did not flower yet. In this paper, we highlight the importance of parental genotype choice related to breeding success within Genisteae.
Le Gloanic, A., Malécot, V., Belin, J., Heinry, E. and Kapusta, V. (2015). INTERSPECIFIC HYBRIDIZATION IN THE TRIBE GENISTEAE USING IN VITRO EMBRYO RESCUE. Acta Hortic. 1087, 315-320
Cytisus scoparius, Cytisus purpureus, postzygotic incompatibility, DNA content