K.S. Nascimento, M.M. Rêgo, A.M.M. Nascimento, E.R. Rêgo
The genus Capsicum comprises economically important species around the world, among them the most cultivated species is Capsicum annuum L., which has great ornamental potential. Methods to create new plant varieties are the objectives of breeders’ studies. An interesting method is the induction of mutation. Among the mutagens used, ethyl methanesulfonate (EMS) is known for being less phytotoxic and for its high efficiency. Given these facts, the objective of the present study was to analyze the effect of EMS in ornamental pepper (C. annuum L.). Seeds were soaked in distilled, deionized and autoclaved water for 12 h, and then subjected to different EMS treatments: 7 concentrations (0.0, 0.025, 0.050, 0.10, 0.15, 0.20, and 0.25%) and 2 exposure times (3 and 6 h), resulting in fourteen treatments. Seeds were in vitro sown on Murashige and Skoog based medium and in the seedlings stage they were acclimated and transferred to a greenhouse. No significant differences were observed for variables germination and seedling height. Of the eighteen traits evaluated in the plant stage (leaves, flowers and fruit), the following thirteen showed significant differences: petal diameter, style length, fruit weight, fruit length, largest and smallest fruit diameter, pedicel length, pericarp thickness, placental length, number of seeds, fruit fresh matter, fruit dry matter, and fruit dry matter content, which indicates that EMS generated variations in plants/fruits from seeds treated with ethyl-methanesulfonate. In this study, the EMS dose (0.1%) and the 3 h exposure time were responsible for the major variations in the traits, so they can be used in breeding programs of this species.
Nascimento, K.S., Rêgo, M.M., Nascimento, A.M.M. and Rêgo, E.R. (2015). ETHYL METHANESULFONATE IN THE GENERATION OF GENETIC VARIABILITY IN CAPSICUM. Acta Hortic. 1087, 357-363
DOI: 10.17660/ActaHortic.2015.1087.47
EMS, mutagen, peppers

Acta Horticulturae