A PROTEOMIC APPROACH TO INVESTIGATE THE ACTINIDIA SPP./PSEUDOMONAS SYRINGAE PV. ACTINIDIAE PATHOSYSTEM
Pseudomonas syringae pv. actinidiae (Psa), the causal agent of the bacterial canker of kiwifruit, uses leaves as an entry site to colonize plants. Upon leaf attack, this Gram-negative bacterium reaches systemically the plant shoot in a week. A combined approach based on two-dimensional gel electrophoresis (2-DE) and nano-scale liquid chromatographic electrospray ionization tandem mass spectrometry (nanoLC-ESI-LIT-MS/MS) was used to describe the major proteomic changes in Actinidia deliciosa Hayward leaf apoplast during the bacterial biotrophic phase and in A. chinensis Soreli shoot following bacterial inoculation in host leaf. We identified a concerted action of different proteins. The proteins involved in plant defence, including type-members of the plant basal defence, pathogenesis, oxidative stress and heat shock, and those involved with transport and signaling events, were the most represented category of induced components. Concentration of proteins involved in carbohydrate metabolism and photosynthesis were also increased upon infection. These proteins intervened at different times and participated in reducing the pathogen population. In parallel, a bacterial outer membrane polypeptide component was identified in shoot tissues, while in leaf apoplast colonization we found other proteins such as bacterial BamA highly represented during the first week, OmpA and Cpn60 induced later. In addition to presenting further proteomic information on the molecular factors actively participating in this pathosystem, our data characterize several events of host colonization by Psa and will promote the eventual development of novel bioassays for pathogen detection in kiwifruit plants.
Petriccione, M., Di Cecco, I., Salzano, A.M., Arena, S., Scaloni, A. and Scortichini, M. (2015). A PROTEOMIC APPROACH TO INVESTIGATE THE ACTINIDIA SPP./PSEUDOMONAS SYRINGAE PV. ACTINIDIAE PATHOSYSTEM. Acta Hortic. 1095, 95-101
kiwifruit, leaf apoplast, systemic infection, biotic stress, plant defence mechanisms