Cloning and characteristics of flavonol synthase promoter from Malus crabapple
A 1.8-kb 5RSQUO-upstream sequence of flavonol synthase (McFLS) from LSQUOever-red-leafRSQUO color types of Malus crabapple cultivar LSQUORoyaltyRSQUO was isolated. The PLACE database (a database of motifs found in plant cis-acting regulatory DNA elements) analysis showed that there were numerous functional elements in the McFLS promoter. Many light-responsive elements that indicated the expression of McFLS had a close relationship with light induction was discovered. Abundant auxin and gibberellic acid (GA) responsive elements suggested that McFLS mainly expressed in the early development stage. Many stress-responsive elements were consistent with the functional characteristics of flavonols in induction and stress-resistance reactions. The expression level analysis verificated the role of cis-element in McFLS promoter. To conclude, the McFLS promoter has potential research significance to explore the function of McFLS.
Zhang, J., Tian, J., Song, T. and Yao, Y. (2015). Cloning and characteristics of flavonol synthase promoter from Malus crabapple. Acta Hortic. 1106, 123-130
FLS, promoter, Malus crabapple, cis-element