In vitro propagation of Lippia citriodora

In vitro cultures were established from node explants excised from adult plants and cultured on MS medium with 30 g L^-1 sucrose, supplemented with N⁶-benzyladenine (BA) and naphthalene acetic acid (NAA) at various concentrations. At the establishment stage the highest response for shoot production was recorded on MS medium supplemented with 1.0 mg L^-1 BA and 0.01 mg L^-1 NAA. At two following subcultures higher BA concentration, as well as zeatin (ZEA), were tested as for their effect on shoot multiplication. The increase of BA concentration from 1 to 2 mg L^-1 did not affect multiplication; neither did the use of 2 mg L^-1 ZEA instead of BA in terms of multiplication index. Similarly to the establishment stage, the combination 1 mg L^-1 BA/ 0.01 mg L^-1 NAA induced good response, giving 2-3 shoots explant^-1 with 3 nodes shoot^-1, that allowed successful continuation of the in vitro culture and quite good shoot proliferation. Microshoots rooted at 67% on MS medium with 1 mg L^-1 IBA. Ex vitro acclimatization was successful (83%) on a mixture of peat:perlite (1:1, v/v).