Features of canna regeneration in vitro and plantlets adaptation in vivo
Using in vitro culture for propagation of canna (Canna × hybrida hort.) gives us the possibility to rapidly multiply virus-free stock plant material. We have studied features of in vitro regeneration ability and in vivo adaptation of 3 canna cultivars ('Dar Vostoka', 'Livadia', 'Suevia'), grown at the collection plot of Nikita Botanical Gardens (NBG). Surface-sterilized vegetative buds were cultured on solidified half-strength Murashige and Skoog (1962) medium (MS), supplemented with cytokinins (thidiazuron NDASH TDZ, 6-benzylaminopurine NDASH BAP) and auxins (3-indoleacetic acid NDASH IAA, 1-naphthalene acetic acid NDASH NAA). Adventitious shoot formation on the MS medium with 1.24-1.91 mg L-1 TDZ has been shown for all the tested cultivars. The multiple meristemoids have been regenerated at the base of adventitious microshoots after its separation and subculture on a fresh medium. After 6-7 months, 20 and 40 meristemoids (size 0.1-0.7 cm) per explants have been formed for each cultivar. To regenerate the plants, the meristemoids were placed on the MS medium supplemented with 2-4 mg L-1 BAP or 1.5 mg L-1 BAP and 1.5 mg L-1 IAA. 100% of 'Suevia' microshoots rooted on MS medium with 1.25 mg L-1 IAA or 1.5 mg L-1 NAA. The regenerants with 2-3 leaves and 5-6 roots have been transplanted to adaptation in vivo. At the beginning of the adaptation 'Suevia' plantlets demonstrated a higher survival ability in vivo compared to regenerants of 'Livadia'. The best results of adaptation have been reached due to the apply of a mixture of leaf mold and agroperlite (survival NDASH 75%), compared with simply perlite (50%). Investigation of morphoanatomical structure of leaves and redistribution of fractional water composition has showed an adaptability in vivo of regenerated 'Suevia' and 'Livadia' plantlets.
Mitrofanova, I.V., Tevfik, A.Sh., Mitrofanova, O.V., Brailko, V.A. and Lesnikova-Sedoshenko, N.P. (2017). Features of canna regeneration in vitro and plantlets adaptation in vivo. Acta Hortic. 1155, 447-454
Canna × hybrida, fluorescence, meristemoid, regenerant, stomata index, vegetative bud