Effects of media and hormones on the adventitious shoot regeneration and development of Manchurian ash

Embryo hypocotyls from mature seeds of Manchurian ash (Fraxinus mandshurica) were used as explants to study the in vitro culture of the species. Adventitious buds were obtained on the tested four media of MS, 1/2MS, WPM and DKW, and the induction rate reached 100% when explants were cultured on MS + TDZ 1.0 mg L^-1 and WPM + TDZ 0.1 mg L^-1. The mortality of explantswas high on MS after 4 weeks of culture but lower on WPM (<10.5%) during the whole culture period. From the more general point of view, WPM is better than MS for adventitious bud induction and growth. The better media and hormone combinations for induction, elongation and rooting of the adventitious buds are WPM containing TDZ 0.1~0.2 mg L^-1, BA 2 mg L^-1 and IBA 0~0.01 mg L^-1, and IBA 0~0.1 mg L^-1, respectively. The rooted shoots were then successfully acclimatized in a greenhouse.