Anticlastogenic effects of organically grown moringa (Moringa oleifera), lemongrass (Cymbopogon citratus), and sweet sorghum (Sorghum bicolor) using micronucleus test
This study was conducted to determine the anticlastogenic effects of expressed juice of moringa (Moringa oleifera Lam.), lemongrass (Cymbopogon citratus), and sweet sorghum (Sorghum bicolor) on rats (Rattus norvegicus). Twenty five female albino rats aged two months were equally allotted in five treatments. The five treatments were: tetracycline as the positive control, distilled water as the negative control, moringa, lemongrass, and sorghum. Initial body weight and vital signs namely: heart rate, respiratory rate and body temperature were recorded prior to the application of the treatment. During the treatment period of four weeks, R. norvegicus rats were provided extracts of moringa, sweet sorghum and lemongrass at a dosage rate of 0.5 mL 20 g-1 of body weight (bw). The test animals were sacrificed 6 h following the last oral administration of tetracycline and were subjected to micronucleus test. Results revealed that moringa, lemongrass, and sweet sorghum have the potential to significantly reduce the number of micronucleated polychromatic erythrocytes (MPCEs) in clastogen-induced rats. Moringa exhibited the least mean number of MPCEs (3.8), followed by sweet sorghum (5.8) and lemongrass (7.8). The positive control showed the highest mean number of MPCEs (37.2). In terms of body weight, vital signs, feed intake and water intake, statistical analysis revealed that moringa, lemongrass, and sorghum at the level of treatment (0.5 20 g-1 bw) did not elicit any adverse effects such as tachycardia, arrhythmia and hyperpnea after a period of four weeks of treatment. The findings of this study suggest that moringa, sweet sorghum and lemongrass have anticlastogenic activities as shown by their ability to shield the interaction of tetracycline with the base pairs of deoxyribonucleic acid, thereby preventing the chromosomal breaking activity of tetracycline. However, further studies on the chemical constituents present in these medicinal plants need to be further investigated. Moreover, the use of mitomycin as clastogenic drug is considered for future studies.
Sanchez, G.C., Dizon, M.D. and Soriano Jr., H.M. (2017). Anticlastogenic effects of organically grown moringa (Moringa oleifera), lemongrass (Cymbopogon citratus), and sweet sorghum (Sorghum bicolor) using micronucleus test. Acta Hortic. 1158, 249-256
anticlastogen, genotoxin, lemongrass, moringa, micronucleus test