Cytotoxicity effect of water extracts of Moringa oleifera leaves and seeds against MCF-7 cells
Glucosinolates are the most abundant compounds in Brassicaceae, Moringaceae and Caricaceae. The glucosinolates will be hydrolysed to their degradation products by myrosinase when plant tissues are disrupted. Water extracts of Moringa oleifera leaves (MOL) and seeds (MOS) were screened for cytotoxic activity against breast adenocarcinoma (MCF-7) cells. Benzyl isothiocyanate (BITC) which possesses a cytotoxic effect on MCF-7 cells was evaluated by using AlamarBlue® assay. The water extract of MOL and MOS showed significant potential to reduce the viability of MCF-7 cells with inclusive 50% inhibition concentration (IC50) at each time point of the treatments (IC50 of MOL: 25.3 µg mL-1 at 24 h; 18.8 µg mL-1 at 48 h; and 6.25 µg mL-1 at 72 h) (IC50 of MOS: 36.4 µg mL-1 at 24 h; 24.1 µg mL-1 at 48 h; and 19.3 µg mL-1 at 72 h). This anticancer activity corresponded with the morphology of the cells by staining with acridine orange (AO) and propidium iodide (PI) where the treated MCF-7 cells showed typical criteria of cell death. P38 mitogen-activated protein kinase (MAPK) was detected using Western blot technique and our results have shown that p38 protein expression of treated MCF-7 with MOL and MOS extract was upregulated after 24 h and down-regulated after 48 h, suggesting that the cell death event was mediated by p38-MAPK protein.
Rahaman, M.H.A., Kadir, N.H.A., Amin, N.M. and Omar, W.B.W. (2017). Cytotoxicity effect of water extracts of Moringa oleifera leaves and seeds against MCF-7 cells. Acta Hortic. 1158, 279-286
cytotoxicity, p38 protein expression, benzyl isothiocyanate