Effect of ethylene precursor 1-aminocyclopropane-1-carboxylic acid and ethylene inhibitor, silver thiosulfateon organogenesis of PLBs in Dendrobium kingianum cultured in vitro
This study attempted to clarify the effects of 1-aminocyclopropane-1-carboxylic acid (ACC) and silver thiosulfate (STS) on organogenesis of PLBs in Dendrobium kingianum under white fluorescent lamps in vitro. PLBs were cultured in modified Murashige and Skoog (MS) medium supplemented with ACC at 0, 0.1, 1 and 10 mg L-1 for two days then transferred and combined with STS at 0, 0.01, 0.1 1 and 10 mM. The highest number of PLBs (12.9) was recorded in the media containing 0.1 mg L-1 ACC combined with 0.01 mM STS. Increase in fresh weight showed higher values in the same combination. Whereas, the highest number of developing shoots (2.7) was recorded in the medium containing 0.1 mg L-1 ACC combined with 0.1mM STS. Low concentration of STS alone increased the number of PLBs but showed inhibitory effects on shoot formation. On the other hand, low concentration of ACC alone increased both number of PLBs and shoots after 4 weeks of culture. In this study, the efficiency of ACC at low concentration was observed only in PLB formation but no satisfactory enhancement on shoot formation.
Habiba, S.U., Shimasaki, K., Ahasan, M.M. and Uddin, A.F.M.J. 2017. Effect of ethylene precursor 1-aminocyclopropane-1-carboxylic acid and ethylene inhibitor, silver thiosulfateon organogenesis of PLBs in Dendrobium kingianum cultured in vitro. Acta Hort. (ISHS) 1167:133-138
Dendrobium, protocorm-like body, 1-aminocyclopropane-1-carboxylic acid, silver thiosulfate