Genetic diversity of some species in the genus Calanthe analyzed by RAPD markers
Genetic diversity among nine accessions of orchids in the genus Calanthe and a hybrid were analyzed by using random amplified polymorphism DNA (RAPD) technique. Twenty out of forty universal primers could amplify in all samples and showed polymorphic bands. Similarity index was calculated and dendrogram was reconstructed using UPGMA clustering method. A total of 204 DNA fragments, ranged from 300 to 2,300 bp, were amplified, of which 198 (97%) were polymorphic. The similarity index was between 0.286 (C. vestita - Calanthe sp. 6) and 0.843 (Calanthe sp. 1-2). A dendrogram consisted of three major clades the coefficient of 0.6, namely, Calanthe sp. 6 clade, C. cardiogrossa and a hybrid clade and the rest samples forming a large clade. In the large clade, there were two subgroups of C. vestita-C. rubens and Calanthe sp.1-5.
Punjansing, T., Chaichanachap, N., Sanitchon, J., Pinta, W. and Nakkuntod, M. (2017). Genetic diversity of some species in the genus Calanthe analyzed by RAPD markers. Acta Hortic. 1167, 323-330
orchid, DNA marker, evolutionary relationship