Cloning and sequence analysis of a PRR5 gene from day-neutral chrysanthemum

Y.H. Gao, Y.K. Gao, X.L. Bu
Most chrysanthemums are short-day plants flowering of which is affected by the light cycle, which seriously restricts year-round production of chrysanthemum. However, Chrysanthemum × morifolium 'Jinbudiao' is a typical day-neutral cultivar, which blooms from May to November without an impact of light length. The continuous-flowering 'Jinbudiao'O usually follows several flowering pathways, similar to Arabidopsis thaliana. However, the most significant ways are the photoperiod pathway and circadian rhythm. cmPRR5 is an important gene in the circadian rhythm pathway in Arabidopsis thaliana. In this study, a PRR5 (named cmPRR5) gene, which is one of the important clock genes, was isolated from Chrysanthemum × morifolium 'Jinbudiao'. The cDNA of cmPRR5 obtained was 1781 bp, and the open reading frame (ORF) was 1740 bp, encoding a predicted protein of 579 amino acid residues with molecular mass of 141.44 kDa and theoretical Pi of 5.0. GenBank BlastX search revealed that cmPRR5 shared a high degree of homology with proteins of the REC superfamily, and the close relatives of cmPRR5 were EgPRR5 from Erythranthe guttata (52% identity) and VvPRR5 from Vitis vinifera (49% identity). Previous research has shown that the PRR genes in Arabidopsis thaliana play an important role in flower initiation.
Gao, Y.H., Gao, Y.K. and Bu, X.L. (2017). Cloning and sequence analysis of a PRR5 gene from day-neutral chrysanthemum. Acta Hortic. 1171, 187-192
DOI: 10.17660/ActaHortic.2017.1171.25
Chrysanthemum × morifolium, PRR5 gene, clone, sequence analysis, circadian rhythm

Acta Horticulturae