Genetic diversity assessment of Tunisian fig (Ficus carica L.) using conserved DNA-derived polymorphism

S. Haffar, A. Ben Abdelkrim, A. Salhi-Hannachi, G. Baraket
The fig (Ficus carica L., family Moraceae) is a hardy fruit tree, it is one of the oldest cultivated fruit species in the world and it is well adapted to the Mediterranean climate. The CDDP markers (conserved DNA-derived polymorphism) were revealed to review and assess the genetic diversity of wild cultivated and Tunisians fig is important step in any development program and conservation of these resources. For this, three primers were considered and tested. A total of 47 bands were generated with 46 (97.87%) were polymorphic. The resolving power (Rp) was estimated and varied from 6.83 for ERF3 primer to 4.47 for the MADS-4 with an average of 5.30. The polymorphism information content (PIC) was calculated and varied from 0.92 for the ERF3 primer to 0.87 for the MADS-4 primer with a mean of 0.89. These results demonstrate the effectiveness of CDDP primers used in this paper for the molecular polymorphism study in Tunisian fig germplasm. The genetic distances were estimated and it's varied from 0.095 to 0.922 with a mean of 0.646. A neighbor-joining (NJ) phylogenetic tree and the principal components analysis (PCA) were constructed based on the Genetic distances and demonstrate that the classification of the Tunisian fig ecotypes was independently of their geographic origin and dependently of their sex of tree. These results show the power and utility of CDDP markers for the genetic diversity and the phylogenetic relationships studies of Tunisian fig.
Haffar, S., Ben Abdelkrim, A., Salhi-Hannachi, A. and Baraket, G. (2017). Genetic diversity assessment of Tunisian fig (Ficus carica L.) using conserved DNA-derived polymorphism. Acta Hortic. 1173, 51-56
DOI: 10.17660/ActaHortic.2017.1173.9
Ficus carica L., CDDP, NJ, ACP, genetic diversity, Tunisia

Acta Horticulturae