Clonal fidelity and morphological and chemical variations in micropropagated Vaccinium plants
Significant advances in Vaccinium micropropagation have been achieved along with the tremendous progress of plant tissue culture techniques and their acceptance globally by commercial growers. Bioreactor micropropagation in liquid media has evolved as an alternative to conventional micropropagation on semi-solid gelled media, to cut down on propagation costs. However, optimal propagation rate is dependent on biochemical and physiological factors responsible for culture growth in a liquid culture system. Enhanced vegetative growth and change in biochemical components are common in in vitro propagated Vaccinium plants. Somaclonal variation, either genetic, epigenetic or from a combination of both, can affect the scaling up of any micropropagation protocol. DNA-based markers can be used in tissue culture propagated Vaccinium species to monitor clonal fidelity in micropropagated plants. This review describes in short the progress of various aspects of Vaccinium culture in vitro, the variation in micropropagated Vaccinium plants and the use of molecular markers to identify these variations.
Debnath, S.C. (2017). Clonal fidelity and morphological and chemical variations in micropropagated Vaccinium plants. Acta Hortic. 1180, 111-116
bioreactor micropropagation, blueberry, cranberry, lingonberry, molecular markers, somaclonal variation