Proteome analysis of Lilium lancifolium in response to cold stress and abscisic acid induction

Q. Wang, J.M. Wang, Y.Y. Yong, W.Q. Li, Y.Y. Zhang, Y. Zhang, Y.M. Lu
Ecological balance and metabolism activity are caused by changes in the expression of differential genes and proteins when plants are subjected to various stresses. Lilium lancifolium is a very important cold-resistant wild flower for lily cold resistance breeding. In order to study the response mechanism to stresses (cold and ABA) in-depth, a proteome study was conducted on the basis of 2-D gel electrophoresis. One hundred and forty-nine significantly differential protein spots were found by DIGE technology, 21 spots with at least 2-fold altered expressions were analyzed by MALDI-TOF and ESI-MS/MS, and 19 proteins were successfully identified. Moreover, the proteins were classified into 'metabolism process' (35.1%), 'response to stress' (20.7%), 'energy' (12.5%), 'signal transduction mechanism' (10.1%), 'others' (10.0%), 'protein binding' (6.2%), and 'biogenesis of cellular component' (5.4%). The functional classification of proteins was almost in accordance with that of genes by RNA-seq, especially the change and damage to the protein structure caused by low temperature, which could be relieved after being treated with ABA. Our study of differentially expressed proteins associated with metabolic pathways furthered the research of cold-resistance proteins and confirmed the consistency in functional classification between differential genes and differential proteins in L. lancifolium. This will be helpful for further study and deeper research of cold-resistance breeding in lily.
Wang, Q., Wang, J.M., Yong, Y.Y., Li, W.Q., Zhang, Y.Y., Zhang, Y. and Lu, Y.M. (2017). Proteome analysis of Lilium lancifolium in response to cold stress and abscisic acid induction. Acta Hortic. 1185, 163-174
DOI: 10.17660/ActaHortic.2017.1185.22
https://doi.org/10.17660/ActaHortic.2017.1185.22
Lilium lancifolium, proteomics, cold stress, ABA
English

Acta Horticulturae