Regeneration of grapevine virus-free plants by in vitro chemotherapy

I.C. Guţă, E.C. Buciumeanu, L.D. Tătaru, C.M. Topală
Chemical drugs used in human medicine are shown to be effective for treating grapevine virus infections. This is demonstrated under controlled conditions on in vitro cultures. Virus-infected grapevine cultivars (Vitis vinifera L.) were grown for 30-40 days on agarized media supplemented with with 60 mg L-1 ribavirin and 120 mg L-1 oseltamivir viricides, used both individually and in admixture. Regenerated plantlets were indexed by DAS-ELISA. Grapevine fanleaf virus could not be removed from 'Valerien' Grapevine leafroll associated virus 1-free plants 'Frâncuşă 15 Od.' were regenerated at a rate of 26 and 4% after exposure to ribavirin and combined viricides, respectively. The treatment with combined viricides was effective and complete elimination of Grapevine fleck virus infection was observed.
Guţă, I.C., Buciumeanu, E.C., Tătaru, L.D. and Topală, C.M. (2017). Regeneration of grapevine virus-free plants by in vitro chemotherapy. Acta Hortic. 1188, 319-322
DOI: 10.17660/ActaHortic.2017.1188.42
virus-elimination, Vitis, ribavirin, oseltamivir, tissue culture

Acta Horticulturae