The effect of cytokinin type and concentration on micropropagation of Calamintha cretica
Calamintha cretica (f. Lamiaceae) is a perennial herb endemic to West Crete that could be used as an ornamental plant. In the present work the in vitro propagation of the species was studied. At the establishment stage, MS medium supplemented with 1.0 mg L-1 zeatin (ZEA) induced higher shoot production compared to the hormone-free (HF) medium, or that supplemented with 1.0 mg L-1 N6-benzyladenine (BA), in both shoot tip and node explants excised from adult plants (53 and 79% shooting respectively, 3-4 shoots explant-1). At the multiplication stage, the effect of cytokinin type, BA, ZEA, kinetin (KIN), 6-(γ,γ-dimethylallylamino)purine (2iP), and concentration, 0.25, 0.5, 1.0 mg L-1, on shoot production was investigated. The explant response was quite similar in all plant growth regulator-concentration combinations tested and only slightly higher multiplication index was observed in the medium with 0.5 or 1.0 mg L-1 KIN. KIN was also the only plant growth regulator that induced 100% shooting of the explants. Microshoot rooting was highly successful (90%) on HF-half strength MS medium. Ex vitro acclimatization of the microplants was 90% on a mixture of 1 peat:1 perlite (v/v).
Vlachou, G., Papafotiou, M. and Bertsouklis, K.F. (2017). The effect of cytokinin type and concentration on micropropagation of Calamintha cretica. Acta Hortic. 1189, 477-480
herb, explant type, rooting, ex vitro acclimatization, native ornamental