The folk medicine as tool for discovery of new anti-inflammatory drugs: the example of Spermacoce verticillata
In the aqueous extract of leaves (SVL) and stems (SVC) of Spermacoce verticillata (L.) G. Mey. the flavonoids rutin and kaempferol-3-O-rhamnoside, and chlorogenic acid were identified. Oral (p.o.) treatment with SVL and SVC (1 g kg-1) inhibited the leukocyte migration to pleural cavity by 33.7 and 47.9%, respectively when compared to vehicle group on carrageenan-induced pleurisy. Similarly butanol (SVB - 200 mg kg-1), ethyl acetate (SVAe - 25 mg kg-1) and flavonoidic fractions (SVLFL - 20 mg kg-1) of SVL inhibited the leukocyte migration by 68.5, 77.4 and 77.5%, respectively. In the croton oil-induced ear edema and the acetic acid-induced writhing tests, SVL (0.1, 0.3 or 1 g kg-1, p.o.) produced a dose-related inhibition of edematogenic and nociceptive responses, respectively at the high dose. The chemotaxis inhibitory activity of both extracts and the anti-edematogenic and antinociceptive activities of SVL are characteristic effects of anti-inflammatory drugs, and these results highlight the therapeutic potential of S. verticillata against inflammatory diseases. To the best of our knowledge, this is the first report for the anti-inflammatory and antinociceptive activities for S. verticillata.
Lima, I.C., Castro, R.N., Chaves, D.S.A., Ferreira, R.T., Carvalho, M.F., Malvar, D. do C. and Vanderlinde, F.A. (2018). The folk medicine as tool for discovery of new anti-inflammatory drugs: the example of Spermacoce verticillata. Acta Hortic. 1198, 67-74
ethnopharmacology, flavonoids, HPLC, leukocytes, Borreria verticillata