Ultrasound extraction and quantification of tormentic acid by HPLC in Cecropia pachystachya Trec.
The specie Cecropia pachystachya Trec. belongs to Cecropiaceae family and is a tree found in secondary vegetation, which attains height between 5 to 25 m. This species is popularly known as embaúba, umbaúba and embaúva, and its use in traditional medicine is associated with treating respiratory diseases, high blood pressure, glycemic control and as a diuretic. Other studies conducted with the species in our laboratory promoted the isolation and identification of the active ingredient which indicated the antimalarial activity of the plant. The objective of this study was to quantify the active ingredient present (tormentic acid) in the root of C. pachystachya by ultrasound extraction and quantification by HPLC. The dried roots were triturated and sieved (30 or 100 mesh), weighed (500 mg), extracted with 20 mL of solvent (ethanol or acetone) for ultrasound treatment (5 or 20 min) at controlled temperature (25 or 50°C). The extracts were dried, resuspended, filtered through 0.45 µM membrane and analyzed in triplicate by HPLC from the calibration curve (R2=0.9997), by the external standard method C8 column (4.6×150 mm, 5 µM), mobile phase 0.1% acetic acid in water-MeOH (25:75) at 1 mL min-1 and λ=204 nm, where the tormentic acid showed its peak at 6.4 min of retention time. The data were statistically treated to assess which treatment had the highest amount of active ingredient. The smaller particle size of the sieves used was responsible for the large difference presented in quantities of extracted tormentic acid, for allowing greater contact of the material with the extracting solvent.
Souza, I.T., Pereira, C.K.B., Sant¿ana, A.E.G. and Oliveira, B.H. (2018). Ultrasound extraction and quantification of tormentic acid by HPLC in Cecropia pachystachya Trec.. Acta Hortic. 1198, 111-114
Tormentic acid, Cecropia pachystachya, HPLC, ultrassom