Comparative analysis of resveratrol production in plants and in vitro materials of Arachis repens (Handro)
Arachis repens (Handro), commonly known as peanut grass, is used as an ornamental, forage and ground cover in substitution of common grass species. Different bioactive substances have been identified in A. hypogaea, including resveratrol. However, very few studies have been performed with this objective in other species of the genus. The goal of this work was the establishment of in vitro systems for A. repens, aiming at a comparative analysis of resveratrol production in in vitro produced materials and in vivo plants. Nodal, internodal and leaf segments were excised from in vitro plants and cultured on MS medium supplemented with different concentrations of BA, TDZ, IAA and KIN, used alone or in combination. Explants showed distinct responses according to the growth regulator used, including shoot production and callogenesis. The presence of total phenolics, and the antioxidant activity of extracts from different in vivo and in vitro materials were also evaluated. HPLC analysis detected the presence of resveratrol in these materials, and the highest concentration (4.54 and 5.07 µg mg-1) was observed in calluses derived from nodal and internodal segments cultured in the presence of 13.6 µM TDZ.
Garcia, L.C., Garcia, R., Kazan, E., Pacheco, G. and Mansur, E. (2018). Comparative analysis of resveratrol production in plants and in vitro materials of Arachis repens (Handro). Acta Hortic. 1198, 23-26
micropropagation, peanut, phenolics, DPPH