Phytochemical and pharmacological profile of Crocus sativus L. by-products found in Kashmir
Crocus sativus L. (saffron) is being used widely for variety of purposes both in household as well as for medicinal and Industrial purpose. The present study was undertaken to analyze medicinal potentiality of Crocus sativus L. (saffron) by-products i.e. stamens, styles, petals and leaves which are commercially least priced, scientifically unattended and unexplored. Different extracts were obtained using solvents of increasing polarities and some In-vitro medicinal properties of extracts were determined i.e. antioxidant, anti-cancer and antimicrobial along with phytochemical analysis by using standard methods. Phytochemical profiling of extracts were performed using both qualitative and quantitative (HPLC/GC-MS) techniques which showed presence of important phytoconstituents such as steroids, terpenes, coumarins, saponins, tannins, alkaloids, flavanoids, cardiac glycosides etc. Antimicrobial activities were checked using well diffusion assay against bacterial and fungal pathogenic strains i.e. Escherichia coli (MTCC-77), Proteus mirabilis (MTCC-425), Pseudomonas aeruginosa (MTCC-424), Serratia marcescens (MTCC-7103), Malassezia furfur (MTCC-1374), Candida albicans (MTCC-1637), Stachybotrys chartarum (MTCC-2146), Trichophyton rubrum (MTCC-7859). MIC was determined using disc diffusion method and compared with commercial medicines, the results indicated strong antibacterial/antifungal potential of these extracts. Further, extracts showed promising antioxidant activities against ABTS radical, also in vitro anticancer testing was performed against cancer cell line MCF-7 and compared with Normal cell line-L6, the results have showed the possibility of Saffron by-products to act as sources of anti-cancerous agents. Thus, our results indicated that by-products of Saffron could play a role as, efficient, safe, new and natural sources of phytoconstituents for pharmaceutical, food and cosmaceutical industries.
Mir, M.A., Rameashkannan, M.V., Raj, J.A., Malik, A.H. and Rajesh, T.S. (2018). Phytochemical and pharmacological profile of Crocus sativus L. by-products found in Kashmir. Acta Hortic. 1200, 213-226
saffron by-products, antioxidant, anti-cancer, antimicrobial, stamen, leaf, petal