Phenotyping of Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole

B. Durenne, A. Blondel, N. Ducat, O. Pigeon, M.L. Fauconnier, P. Druart
Epoxiconazole like others triazole fungicides are known to be persistent in the soil. Several studies using foliar application experiments demonstrated the effect of its triazole metabolite as plant growth regulator through the anti-gibberellin activity. And notably, the reduction of Brassica napus L. growth can be attributed to the inhibition of gibberellin biosynthesis at the stage of conversion of ent-kaurene to ent-kaurenoic acid. We describe here an in vitro experiment studying the relationship between epoxiconazole in culture medium (0, 0.120 and 0.200 mg L-1) and the phenotyping (root and shoot growth) of three cultivars of winter rapeseeds (Brassica napus L. 'Catalina', 'ES Astrid' and 'Toccata'). Plantlets fungicide content was quantified using the QuEChERS extraction method following by an automated UHPLC-MS/MS analysis. Results showed that the shoots and roots growth of Brassica napus L. plantlets was significantly inhibited by epoxiconazole at 0.120 mg L-1 independently of the cultivar. The concentration of 0.200 mg L-1 leaded to necrosis and anthocyanosis symptoms and can be considered as lethal for in vitro growing explants. The huge epoxiconazole absorption by rapeseed plantlets clearly showed a dose-dependent relationship and was closely similar for the three cultivars.
Durenne, B., Blondel, A., Ducat, N., Pigeon, O., Fauconnier, M.L. and Druart, P. (2018). Phenotyping of Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole. Acta Hortic. 1202, 101-106
DOI: 10.17660/ActaHortic.2018.1202.15
https://doi.org/10.17660/ActaHortic.2018.1202.15
triazole, oilseed rape, growth regulation, fungicide absorption
English

Acta Horticulturae