Negative hydrostatic pressure is an unnoticed but significant source of contamination in tissue culture©

N. Askari, G.-J. De Klerk
Plants are characterized by a negative hydrostatic pressure, brought about by transpiration and by capillary activity of xylem vessels (Taiz and Zeiger, 2010). Because of this, a stem that is being cut sucks up what is nearby. Often this is air but it may also be liquid. The diameter of the xylem vessels is 50-100 μm, so when the liquid contains bacteria (that are typically 0.5-5.0 μm), they will enter deeply into the tissue (Askari et al., 2014; De Klerk et al., 2014). To our knowledge, this alleged source of contamination has never been examined.
Askari, N. and De Klerk, G.-J. (2018). Negative hydrostatic pressure is an unnoticed but significant source of contamination in tissue culture©. Acta Hortic. 1212, 85-88
DOI: 10.17660/ActaHortic.2018.1212.17
https://doi.org/10.17660/ActaHortic.2018.1212.17
English

Acta Horticulturae