Optimising polysaccharide extraction from the spore of Vietnamese lingzhi (Ganoderma lucidum) via enzymatic method
Ganoderma lucidum (an Oriental fungus, known as lingzhi) is commonly used in Vietnam and other Asian countries with excellent beneficial health effects. Lingzhi has potential applications as a functional product with polysaccharides and many other healthy components. Lingzhi is a common mushroom in Vietnam, growing almost all year round. The spore of this mushroom contains high levels of healthy polysaccharides. The polysaccharide extraction from spores of lingzhi was analysed and the enzymatic method of extraction was optimised. The experiment was conducted with the following variables: spore of lingzhi-to-waterratio (S/W, 2:40-2:80); solvent temperature (ST, 40-60°C); enzyme concentration (EC, 1.7-2.9%), and extraction time (ET, 60-180 min). Spores of lingzhi were mixed with the solvent, treated with enzyme, filtered and evaluated for polysaccharide contents. The polysaccharide extraction parameters were optimised. The results showed that the best extraction conditions were S/W, 2:70; ST, 50°C; EC, 2.3%; and ET, 150 min. At such conditions, 6.7285% polysaccharide was extracted. The method is simple and can be applied readily in industry.
Dam, Sao-Mai and Vo, Trung-Au (2018). Optimising polysaccharide extraction from the spore of Vietnamese lingzhi (Ganoderma lucidum) via enzymatic method. Acta Hortic. 1213, 387-392
Ganoderma lucidum, polysaccharide, spore of lingzhi, enzyme, optimising extraction