Detection of Prunus necrotic ringspot virus (PNRSV) in almond and effect on pollen germination

A. Nikbakht Dehkordi, B. Shiran, N. Babaiyan, P. Martínez-Gómez, M. Rubio
Prunus necrotic ringspot virus (PNRSV) is one of the most widespread viruses affecting stone fruit production including almond. By considering negative impact of PNRSV on production of almond, the objective of the present study was to develop a consistent and reliable method to detect PNRSV for studying the effect on pollen germination in the most important almond cultivars grown in Saman region in Chahar Mahal va Bakhtiari province in Iran. One hundred almond trees of ‘Mamaie’, ‘Rabii’ and ‘Sefid’ cultivars were randomly selected during the months of March, June and September of years 2008 and 2009. Samples were evaluated by using ELISA and RT-PCR for the presence of PNRSV. Healthy and infected pollen of ‘Sefid’ were germinated in vitro. The infection with PNRSV showed a clear effect on pollen germination with a reduction of the germination capacity of 34%. In addition, when compared to the ELISA test, RT-PCR was more sensitive in detecting PNRSV. Detection of PNRSV by ELISA was most sensitive when plant material was collected in March, therefore sampling time was an important factor for detection of PNRSV.
Nikbakht Dehkordi, A., Shiran, B., Babaiyan, N., Martínez-Gómez, P. and Rubio, M. (2018). Detection of Prunus necrotic ringspot virus (PNRSV) in almond and effect on pollen germination. Acta Hortic. 1219, 357-362
DOI: 10.17660/ActaHortic.2018.1219.54
almond, ELISA, pollen germination, PNRSV, RT-PCR

Acta Horticulturae