Breeding programs to transmit Asparagus virus 1 resistance
Garden asparagus (Asparagus officinalis) is highly susceptible to Asparagus virus 1 (AV-1) transmitted by green peach aphids (Myzus persicae). Recent screenings of 44 A. officinalis cultivars and 34 wild relatives for AV-1 resistance exposed 29 AV-1 resistant Asparagus wild relatives. The transmission of the resistance and the development of interspecific hybrids were successfully obtained from different crossing combinations between cultivated A. officinalis and the Asparagus wild relatives A. pseudoscaber, A. amarus, A. maritimus and A. prostratus. Crosses of A. officinalis × A. stipularis and A. officinalis × A. densiflorus were unsuccessful. Interspecific hybrids and backcrossed progenies (BC1, BC2) with garden asparagus were generated by using embryo rescue and in vitro regeneration. Only in a few cases hybrids were obtained directly by germinating seeds. Each progeny was tested for AV-1 resistance and hybridity was verified with molecular and cytological methods.
Plath, S., Krämer, R., Lantos, E. and Nothnagel, T. (2018). Breeding programs to transmit Asparagus virus 1 resistance. Acta Hortic. 1223, 17-24
Asparagus officinalis, wild relatives, Asparagus virus 1, interspecific hybridization, embryo rescue, SSR Marker