Morphophysiological and biochemical characteristics of some Crimean aromatic plants developed in vitro

I.V. Mitrofanova, V.A. Brailko, O.A. Grebennikova, A.E. Paliy, O.V. Mitrofanova
Morphology-anatomical, physiology-biochemical aspects of in vitro plant development in essential oil rose, lavender and lavandin from the collection of the Nikita Botanical Gardens were studied. Microshoots on MS medium with different concentrations of BAP, kinetin, NAA were cultivated and kept in a growth chamber (25±1°C, 16-h photoperiod, 37.5 μmol m-2 s-1). To assess microshoots development they were collected after 8-9 month culture. The average length of microshoots in essential oil rose cultivars was 2.4-4.8 cm and in lavender and lavandin – 3.8-4.5 cm. The leaf blades were bifacial, thinner in essential oil rose (79-122 μm) and demonstrated maximum thickness in lavandin cultivars (168-190 μm). The leaf cover tissues were mature and cutinized, mesophyll was differentiated. The essential oil drops were found in epidermis and mesophyll. The indexes of photosynthetic activity ranged 0.28-0.61 a.u. The highest viability indexes characterized the rose cultivar ‘Festivalnaya’ (2.57 a.u.) and lavender ‘Rabat’ (2.36 a.u.). The total water content was higher in rose cultivars (87-89%). The part of bound water was greater in the lavender cultivars. Perhaps it depends on a proline accumulation (up to 35.72 μg g-1) and much more active synthesis of antioxidants – ascorbic acid and phenolic compounds. Catalase activity was higher in essential oil rose cultivars (10-16 g O2 g-1 min-1), SOD activity in all the studied plants was 5-13 a.u g-1, PPO activity – 0.061-0.183 a.u. g-1 s-1. The maximum morphogenetic capacities were in essential oil rose cultivar ‘Festivalnaya’, lavender ‘Record’ and lavandin ‘Rabat’.
Mitrofanova, I.V., Brailko, V.A., Grebennikova, O.A., Paliy, A.E. and Mitrofanova, O.V. (2018). Morphophysiological and biochemical characteristics of some Crimean aromatic plants developed in vitro. Acta Hortic. 1224, 101-108
DOI: 10.17660/ActaHortic.2018.1224.14
essential oil, rose, lavender, lavandin, regenerant, structural features, photosynthetic activity, antioxidants

Acta Horticulturae