Microsatellites as a tool for identifying successful pollinators of the pear cultivar 'Ingeborg' in Ullensvang, Norway
Due to their Mendelian inheritance, microsatellites or SSRs (simple sequence repeats) can readily be used for parentage analyses of pear seedlings, thus revealing the female and male parents of the plant. However, in cases where obtained pear seeds display low viability, conducting a parentage analyses on the pear seeds themselves could be used in order to identify the male parent (successful pollinator). In this study, batches of seeds were extracted from fruits of the triploid Ingeborg pear (Conference × Bonne Louise), the main commercial pear cultivar in Norway. Mature pears were collected from eight commercial pear orchards located in Ullensvang, at 60° North and seeds were collected. Genomic DNA was subsequently isolated from the obtained seed batches and genotyped using 12 microsatellite markers. The same markers were also used for the genetic characterization of Ingeborg and five pear genotypes used as pollinators in the sampled orchards (Clara Frijs, Herzogine Elsa, Anna, Colorée de Juillet and Belle lucrative). The obtained SSR profiles were used in paternity analyses, as well as for gene assignment analyses. Both approaches identified pear Clara Frijs to be the most successful pollinator in most of the sampled orchards. Subsequent S allele genotyping of Ingeborg and five pear genotypes used as pollinators in the sampled orchards revealed that the preferential pollination by individual genotypes was not caused by gametophytic incompatibility or semi-compatibility.
Meland, M., Kurtovic, M., Kalamujic, B., Pojskic, N., Lasic, L. and Gasi, F. (2018). Microsatellites as a tool for identifying successful pollinators of the pear cultivar 'Ingeborg' in Ullensvang, Norway. Acta Hortic. 1229, 57-60
Pyrus communis L., pear seeds, gene assignment analyses, gametophytic incompatibility