S-genotyping of Hungarian sour cherry cultivars
Sour cherry (Prunus cerasus L.) is native to Hungary and some Hungarian cultivars are grown worldwide. Sour cherry includes both self-incompatible and self-compatible accessions. Being an allotetraploid spontaneous hybrid of sweet and ground cherries with 32 chromosomes in sporophytic cells, the S-allele pools of sweet and sour cherries are expected to overlap. SC is primarily due to inactive S-haplotype-specific F-box (SFB) protein in diploid pollen carrying two non-functional S-haplotypes. We determined the S-allele composition of 32 Hungarian sour cherry cultivars using intron length polymorphism, allele-specific PCR, CAPS markers and DNA sequencing. We found a total of 10 S-haplotypes (S1, S4, S6, S9, S12, S13, S14, S26, S35 and S36) or their non-functional variants. It was the first time to use the first intron length analysis for S-allele identification in sour cherry. Both S-genotype and simple sequence repeat fingerprinting confirmed that Pipacs 1 and other famous amarelle-type sour cherries (Pándy and Montmorency) share no common ancestry. Pipacs 1 is a unique cherry cultivar exclusively accumulating considerable amounts of isoflavonoid compounds in fruit. Hence its molecular differentiation from other amarelle-type cherries may be of great importance.
Halász, J., Balogh, E., Makovics-Zsohár, N. and Hegedűs, A. (2019). S-genotyping of Hungarian sour cherry cultivars. Acta Hortic. 1231, 161-166
CAPS, S-haplotype, fingerprinting, intron length polymorphism, Prunus cerasus