The viral complex associated with mealybug wilt disease of pineapple in Cuba

L. Hernández-Rodríguez, P.L. Ramos-González, V. Sistachs-Vega, V. Zamora-Rodríguez, L. Batista-Le Riverend, M. Ramos-Leal, I. Peña-Bárzaga, Y. Llanes-Alvarez
Mealybug wilt of pineapple (MWP) disease has been associated with the infection by a complex of ampeloviruses (Pineapple mealybug wilt-associated virus 1 (PMWaV-1), PMWaV-2, PMWaV-3, PMWaV-4 and PMWaV-5)) (Closteroviridae) and badnaviruses (Pineapple bacilliform CO virus (PBCOV) and Pineapple bacilliform ER virus (PBERV) (Caulimoviridae)). In Cuba, PMWaV-1, PMWaV-2, PMWaV-3 and PBCOV are widespread in commercial pineapple fields, but the association of these viruses with the aetiology of the disease remains unknown. During 2009 to 2012, a survey to detect PMWaV-1, PMWaV-2 and PMWaV-3 by RT-PCR, and PBCOV by non-radioactive Dot Blot hybridization was carried out on samples collected from asymptomatic or MWP symptomatic pineapple plants from 24 commercial fields in 10 provinces and Isla de la Juventud. PMWaV-1, PMWaV-2, PMWaV-3, and PBCOV occurred in simple or mixed infections, whereas PMWaV-2 was the viral species most frequently detected in symptomatic plants. The positive or negative diagnostic results of the RT-PCR to detect PMWaV-2 was more reliably associated with the MWP symptomatic or asymptomatic status of the sampled plants, respectively, than those from PMWaV-1, PMWaV-3 or PBCOV detection techniques. This work discloses the correlation between the infection by PMWaV-2 and the presence of MWP symptoms in Cuba, supporting previous studies indicating the major role of this virus triggering MWP disease.
Hernández-Rodríguez, L., Ramos-González, P.L., Sistachs-Vega, V., Zamora-Rodríguez, V., Batista-Le Riverend, L., Ramos-Leal, M., Peña-Bárzaga, I. and Llanes-Alvarez, Y. (2019). The viral complex associated with mealybug wilt disease of pineapple in Cuba. Acta Hortic. 1239, 203-212
DOI: 10.17660/ActaHortic.2019.1239.25
ampelovirus, badnavirus, MWP, Ananas comosus, Closteroviridae, Caulimoviridae, PMWaV, PBCOV

Acta Horticulturae