New approach of electrotherapy for grapevine virus elimination
Electrotherapy in a horizontal electrophoresis tank was applied to remove single and mixed virus infections in grapevine cultivars (Vitis vinifera L.). Electric stimulation of grapevine herbaceous cuttings with electric current of 40-100 mA, for 5-20 min treatment time was followed by in vitro regeneration of new plants.
ELISA negative grapevines were obtained in different percentages, depending on the virus infection type and experimental parameters.
While the Grapevine fleck virus was removed up to 100%, Grapevine fanleaf virus-free plants were obtained between 44 and 50%, and the lowest virus elimination rate of 25-30% was registered for Grapevine leafroll associated virus type 1. The complex consisting of Grapevine fleck virus and Grapevine virus A was eliminated in 50% of recovered plants.
Guţă, I.-C., Buciumeanu, E.-C., Tătaru, L.D., Oprescu, B. and Topală, C.M. (2019). New approach of electrotherapy for grapevine virus elimination. Acta Hortic. 1242, 697-702
DOI: 10.17660/ActaHortic.2019.1242.103
https://doi.org/10.17660/ActaHortic.2019.1242.103
DOI: 10.17660/ActaHortic.2019.1242.103
https://doi.org/10.17660/ActaHortic.2019.1242.103
Vitis, virus, sanitation, electrophoresis tank, tissue culture
English
1242_103
697-702
- Division Plant Genetic Resources and Biotechnology
- Division Temperate Tree Fruits
- Division Temperate Tree Nuts
- Division Vine and Berry Fruits
- Division Ornamental Plants
- Division Vegetables, Roots and Tubers
- Division Physiology and Plant-Environment Interactions of Horticultural Crops in Field Systems
- Division Protected Cultivation and Soilless Culture
- Division Postharvest and Quality Assurance
- Division Precision Horticulture and Engineering