Effect of indolebutyric acid (IBA) and cane position on rooting of rootstock's cuttings
The aim of this study was to determine the effect of different concentrations of indolebutyric acid (IBA) and to evaluate the influence of the part of the cane from which the cuttings were taken on rhizogenesis of rootstocks (1103 Paulsen, SO4, Dogridge) and grapevine cultivar Vidiano (Vitis vinifera L.). Three rootstocks (1103 Paulsen, SO4, Dogridge) with five different auxin treatments (control, 60, 120, 250 and 1000 ppm IBA) were examined under hydroponic conditions. A comparative study of rhizogenesis was performed between grapevine cultivar Vidiano and the three rootstocks, as well as the part of the cane. The cuttings were collected from the vineyard of the Laboratory of Viticulture of the Agricultural University of Athens. It was shown that, in general, the middle and, secondarily, the base part of the cane give the best results concerning the percentage of kalogenesis, the percentage of rhizogenesis, the average diameter of roots, the average number of roots per cutting, the total surface of roots and the total length of roots. Among the concentrations, 120 and 250 ppm showed a high percentage of rooting for the three rootstocks studied (Dogridge, 1103P, SO4) and the cultivar Vidiano. The effect of three factors (cultivar, part of cane from which the cuttings were taken, IBA concentration) was evaluated concerning the percentage of kalogenesis, the percentage of rhizogenesis, the average diameter of roots, the average number of roots per cutting, the total surface of roots and the total length of roots. The results showed that controls had the shortest roots and 60 ppm IBA showed a sharp increase at root length probably due to increased rhizogenesis compared to that of the controls. The bases of the cuttings of the three rootstocks gave the longest roots probably due to better nutrition. The highest percentages of rhizogenesis were observed in the base, middle and top cuttings of 1103 Paulsen at the treatment of 250 ppm (95, 80, 80%, respectively), followed by the treatment of 120 ppm of the middle cuttings of the same rootstock (75%). The experiment showed that the cultivar (Vidiano) and the rootstocks (1103 Paulsen, SO4, Dogridge) which rooted easily need smaller concentrations of IBA. The treatment of 1000 ppm IBA gave the lowest rates (kalogenesis, rhizogenesis, root number, root length, root surface, and average root diameter) at all cuttings of Vidiano and the rootstocks SO4 and Dogridge.
Daskalakis, I., Biniari, K. and Bouza, D. (2019). Effect of indolebutyric acid (IBA) and cane position on rooting of rootstock's cuttings. Acta Hortic. 1242, 767-774
Vitis vinifera L., rootstocks, cutting, indole-3-butyric acid (IBA), rhizogenesis, hydroponic system