Effect of various culture media on in vitro propagation of grapevine cultivars 'Giouroukiko' and 'Serifiotiko' (Vitis vinifera L.)

The objective of this work was to evaluate the effect of different culture media and the influence of the position of nodal segments on blastogenesis and rhizogenesis, on the in vitro propagation of grapevine cultivars ‘Giouroukiko’ and ‘Serifiotiko’ (Vitis vinifera L.) that are preserved in the Ampelographic Collection of the Agricultural University of Athens. For the in vitro propagation, the culture media MS (Murashige and Skoog), MS modified and WPM (woody plant medium), full or half strength were used. The explants, one-node-cuttings, came from the base, the middle and the top part of the main shoots. The results showed that: a) the position of nodal segments affect the blastogenesis, while the cultivar also plays a significant role. In grapevine cultivar ‘Giouroukiko’, the explants of the base part of the shoot showed the highest percentage of blastogenesis, while in the case of grapevine cultivar ‘Serifiotiko’, it was the explants of the middle part of the shoot; b) regarding the formation of adventitious roots no statistically significant differences between the three categories of explants were observed, although the average number of roots per explant was higher in the explants of the base part of the shoot in both cultivars; c) regarding the six different culture media used, WPM 100% gave the best results for ‘Giouroukiko’ while MS modified 50% for ‘Serifiotiko’; and d) regarding the rhizogenesis induction, no statistically significant difference was observed between the two cultivars and the culture media used.