Identification of downy mildew resistance genes Rpv10 and Rpv3 by DNA-marker analysis in a Russian grapevine germplasm collection
Downy mildew, caused by Plasmopara viticola, is one of the most common fungal diseases of grapevine. To date, more than 20 major and minor resistance loci to P. viticola have been identified. Current work is focused on the search for donors of resistance to downy mildew genes of Rpv10 (inherited from Vitis amurensis Rupr.) and Rpv3 (originating from North American species) according to DNA-marker analysis data. Most of the studied genotypes are interspecies cultivars of breeding from different regions, mostly Russian and post-Soviet Union territory selection. Cultivars that have known identified allelic status for the studied genes were included in the research as reference genotypes. Genotyping was carried out by PCR; PCR products were separated and estimated by capillary electrophoresis using the automatic genetic analyzer ABI Prism 3130. Gene Rpv3 has seven haplotypes of resistance (Rpv3299-279, Rpv3null-297, Rpv3321-312, Rpv3null-271, Rpv3361-299, Rpv3299-314, Rpv3null-287), which can be identified by DNA markers UDV305 and UDV737. In the current study, Rpv3 was detected in 19 cultivars; haplotypes Rpv3299-279, Rpv3321-312 and Rpv3null-271 were found. To determine the presence of the Rpv10 locus, we used a closely linked microsatellite marker, GF 09-46. An allele GF 09-46 that correlates with the presence of a the Rpv10 locus was found in nine accessions.
Ilnitskaya, Е., Tokmakov, S., Makarkina, M. and Suprun, I. (2019). Identification of downy mildew resistance genes Rpv10 and Rpv3 by DNA-marker analysis in a Russian grapevine germplasm collection. Acta Hortic. 1248, 129-134
DNA markers, downy mildew resistance, Rpv10, Rpv3