Application and optimization of the CRISPR/Cas9 system in grape
As an innovative and powerful tool for genome engineering, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been quickly applied to a variety of plant species, including several fruit crops. Through targeted and accurate gene modifications, it enables the development of novel plant cultivars with preferable traits, providing an alternative to plant breeding. However, many factors such as guide RNA (gRNA) and Cas9 protein impact on its efficiency and specificity, which is a major concern of the CRISPR/Cas9 system during genome editing. Here, we summarize accomplishments and problems of genome editing mediated by CRISPR/Cas9 in fruit crops, and discuss recent achievements in optimizing the efficiency and specificity of the system. Accordingly, we provide a basic strategy for genome editing in grape, expecting to hasten the exploration and application of this technology in this species as well as other fruit crops.
Ren, C., Gathunga, E.K., Li, S. and Liang, Z. (2019). Application and optimization of the CRISPR/Cas9 system in grape. Acta Hortic. 1248, 149-154
application, optimization, CRISPR/Cas9, grape, genome editing, efficiency, basic strategy