Whole-genome sequencing and gene annotation of Georgian grape cultivars

V. Tabidze, I. Pipia, M. Gogniashvili, N. Kunelauri, T. Beridze
The genomes of four Georgian grape cultivars, ‘Chkhaveri’, ‘Saperavi’, ‘Meskhetian green’, and ‘Rkatsiteli’, belonging to different haplogroups, were resequenced. Shotgun genomic libraries of grape cultivars were sequenced on an Illumina HiSeq. The sequences of all 19 chromosomes of the four Georgian grape cultivars were determined by resequencing using ‘Pinot noir’ as a reference genome. Annotation of chromosomal DNA of Georgian grape revealed 17,409 candidate genes in ‘Chkhaveri’, 17,021 in ‘Saperavi’, 18,355 in ‘Meskhetian green’ and 13,960 in ‘Rkatsiteli’. Among them, 106 predicted genes and 43 pseudogenes of terpene synthase (TPS) and 44 candidate genes of stilbene synthase (STS) were found in all studied Georgian cultivars. Several additional TPS and STS genes not present in the reference ‘Pinot noir’ genome were detected. This is the first attempt at a comparative whole-genome analysis of different haplogroups of Vitis vinifera cultivars.
Tabidze, V., Pipia, I., Gogniashvili, M., Kunelauri, N. and Beridze, T. (2019). Whole-genome sequencing and gene annotation of Georgian grape cultivars. Acta Hortic. 1248, 287-294
DOI: 10.17660/ActaHortic.2019.1248.41
https://doi.org/10.17660/ActaHortic.2019.1248.41
grape genome, gene annotation, Illumina sequencing, terpene synthase, stilbene synthase
English

Acta Horticulturae