Whole-genome sequencing and gene annotation of Georgian grape cultivars
The genomes of four Georgian grape cultivars, Chkhaveri, Saperavi, Meskhetian green, and Rkatsiteli, belonging to different haplogroups, were resequenced. Shotgun genomic libraries of grape cultivars were sequenced on an Illumina HiSeq. The sequences of all 19 chromosomes of the four Georgian grape cultivars were determined by resequencing using Pinot noir as a reference genome. Annotation of chromosomal DNA of Georgian grape revealed 17,409 candidate genes in Chkhaveri, 17,021 in Saperavi, 18,355 in Meskhetian green and 13,960 in Rkatsiteli. Among them, 106 predicted genes and 43 pseudogenes of terpene synthase (TPS) and 44 candidate genes of stilbene synthase (STS) were found in all studied Georgian cultivars. Several additional TPS and STS genes not present in the reference Pinot noir genome were detected. This is the first attempt at a comparative whole-genome analysis of different haplogroups of Vitis vinifera cultivars.
Tabidze, V., Pipia, I., Gogniashvili, M., Kunelauri, N. and Beridze, T. (2019). Whole-genome sequencing and gene annotation of Georgian grape cultivars. Acta Hortic. 1248, 287-294
grape genome, gene annotation, Illumina sequencing, terpene synthase, stilbene synthase