Proteome analysis to identify anthocyanin transporters in grape cells
Grape accumulates various metabolites, including sugars, organic acids, anthocyanins, tannins and resveratrol. These metabolites determine the taste and quality of the grape berry and wine. Anthocyanins are among the most important metabolites in grape berries, because they control berry color and are involved in biotic and abiotic stress resistance. In addition, anthocyanins are known as functional compounds that have beneficial effects on human health. Anthocyanins accumulate mainly in vacuoles. The present study investigated anthocyanin transporters of the vacuolar membrane through proteome analysis. Two different grape cell lines, VR and VW, were used as materials. VR grown under light (VR-Light) accumulates high amount of anthocyanin, whereas VW grown under light (VW-Light) and VR grown under dark (VR-Dark) did not accumulate anthocyanins. Proteome analysis of vacuolar membrane fractions from VR-Light, VW-Light and VR-Dark allowed us to identify a total of 378 proteins. By comparing the proteins identified among VR-Light, VR-Dark and VW-Light, 16 proteins were suggested as candidates for anthocyanin transporters, and three of them were known anthocyanin transporters. This shows that the proteomic approach performed in this study is effective to identify anthocyanin transporters.
Taki, M., Sakamoto, H., Harada, R., Fukao, Y., Mori, T., Nakabayashi, R., Saito, K. and Shiratake, K. (2019). Proteome analysis to identify anthocyanin transporters in grape cells. Acta Hortic. 1248, 479-486
ABC transporter, anthocyanin transporter, MATE, proteomics, vacuolar membrane