Micropropagation of 'MSXJ' hybrid of papaya in temporary immersion systems
The MSXJ hybrid of papaya (Carica papaya) presents tolerance to temperatures above 35°C, a factor of great importance for the absence of abscission in flowers and fruits. In addition, they emphasize their desirable characteristics of color, smell and sweet taste. A single plant can produce between 32 and 41 elongated fruits without deformations, with a yield of 53-63 kg per plant and field yields of 117-132 t ha‑1. The use of bioreactors under the scheme of temporary immersion systems is an alternative way of reducing costs, increasing biological yield and partially automating the micropropagation of vegetal species. The objective of this work was to determine the efficiency in the micropropagation of MSXJ hybrid of papaya under temporary immersion systems (SETIS® and BIT). Nodal segments of 1 cm were cultured in semisolid MS medium supplemented with 2.2 μM benzylaminopurine (BAP) and 0.2 μM naphthalene acetic acid (NAA), and in semisolid medium with temporary immersion in SETIS® and BIT, with a frequency of a 2-min immersion every 8 h. A completely randomized experimental design with 30 explants per treatment was used. After 30 days, the number and length of shoots were evaluated. The data obtained were subjected to analysis of variance (ANOVA) and Tukeys mean comparison test (p≤0.05). The highest proliferation of shoots per explant was obtained with the implementation of the SETIS system (15.06 shoots), followed by the BIT system (9.8 shoots) and the semisolid medium (6.86 shoots). The longest length was presented in the BIT system (3.42 cm) followed by the SETIS® system (3.07 cm), with the result for semisolid medium being the lowest (0.70 cm). The highest number and length of shoots was found in the BIT and SETIS® temporary immersion systems.
Corona, J., Pastelín, M., Castañeda, O., Solano, L. and Hernadez, K. (2019). Micropropagation of 'MSXJ' hybrid of papaya in temporary immersion systems. Acta Hortic. 1250, 139-144
Carica papaya, bioreactors, in vitro, SETIS®, BIT