Isolation and expression analysis of an ethylene receptor gene Ps-ETR2 in tree peony cut flowers
Tree peony (Paeonia suffruticosa) is a well-known traditional flower with a broad market prospect in China. In order to understand the role of ethylene receptor genes in postharvest development of tree peony cut flowers, an ethylene receptor gene Ps-ETR2 was isolated and analysed in this study. An ethylene-sensitive cultivar Luoyang Hong and an ethylene-insensitive cultivar Xue Ying Tao Hua were selected to analyse the expression pattern of Ps-ETR2 gene in their petals at different developmental stages by quantitative real-time PCR. The results showed that the expression pattern of Ps-ETR2 exhibited significant differences between the two cultivars. During cut flower postharvest development, Ps-ETR2 transcripts in petals of the ethylene-sensitive Luoyang Hong cultivar showed a rapid and obvious rise at the pre-opening stage followed by a gradual decline from pre-opening to full opening stage, and then reached the highest level of mRNA accumulation at senescence. Unlike this pattern, Ps-ETR2 transcripts in petals of the ethylene-insensitive Xue Ying Tao Hua cultivar remained at a relatively low-level during flower opening, and only increased a little when petals wilted. These results suggest that the Ps-ETR2 gene and its resultant protein may be involved in the regulation of ethylene sensitivity of tree peony cut flowers. This may provide a theoretical basis for further research on the molecular mechanism of ethylene-mediated opening and senescence of tree peony cut flowers.
Zhang, L., Wu, F., Zhao, D., Guo, J. and Dong, L. (2019). Isolation and expression analysis of an ethylene receptor gene Ps-ETR2 in tree peony cut flowers. Acta Hortic. 1263, 397-404
Paeonia suffruticosa, cut flowers, ethylene receptor, ethylene sensitivity