New technique for observing mycorrhizal fungi
Mycorrhizal fungi, particularly arbuscular mycorrhizal fungi (AMF) that infect almost all of the plants, play a very important role for the maintenance of natural ecology and the construction of safe, secure and sustainable (3S) crop production. The standard procedure to observe root colonization, however, is cumbersome, and various light microscopical techniques are required to clearly distinguish between AMF and root tissues. Recently, the fluorescein-labeled specific antibody against 24 kDa protein related to mycorrhizal symbiosis was developed. The new antibody was successfully used to detect various kinds of mycorrhizal fungi in fluorescence microscopic observation. In this study, we report on a new technique for observing mycorrhizal fungi by using a newly developed portable fluorescence microscope with the detection reagent.
Ishii, T. and Amanai, K. (2020). New technique for observing mycorrhizal fungi. Acta Hortic. 1267, 297-301
mycorrhizal fungi, mycorrhizal fungus-detection reagent, newly developed portable fluorescence microscope