Development of a robust RNA extraction protocol for black raspberry

C. Mulch, N.V. Bassil, C.E. Finn, M. Dossett, K.J. Vining
Black raspberry (Rubus occidentalis) is a US Pacific Northwest specialty crop prized for its unique flavor profile and nutritional attributes. Market expansion depends on production, which is currently hindered by aphid-vectored viruses, such as Black raspberry necrosis virus. In order to enable dissection of genetic loci controlling aphid resistance, a reliable RNA extraction protocol was necessary to process leaf samples with a range of maturities. We developed and optimized an RNA extraction protocol we refer to as the modified chloroform (MC) method for black raspberry intended for Pacific Biosciences Iso-Seq analysis. Using this method, we were able to produce high quality RNA from mature leaves. Going forward, we will use RNA for identification and fine mapping of quantitative trait loci (QTL) in the black raspberry genome that correlates with the aphid resistance phenotype.
Mulch, C., Bassil, N.V., Finn, C.E., Dossett, M. and Vining, K.J. (2020). Development of a robust RNA extraction protocol for black raspberry. Acta Hortic. 1277, 113-120
DOI: 10.17660/ActaHortic.2020.1277.16
quantitative trait loci (QTL), Iso-Seq, aphid resistance, Rubus occidentalis

Acta Horticulturae