Pestalotiopsis spp. causing grapevine leaf spot and postharvest berry rot in southern Brazil
Pestalotiopsis species were associated with grapevine leaf spot and postharvest fruit rot diseases in many countries worldwide. Pestalotiopsis spp. was isolated from grapevine leaves and berries in 21 municipalities of Rio Grande Sul, Santa Catarina and Parana states, southern Brazil. The isolates were characterized using a combined analysis of morphological features (colony appearances and conidia characters), sequencing the internal transcribed spacer (ITS) region, and pathogenicity test. Five morphological groups were identified based on genetic dissimilarity of coupled conidia size, apical appendage number and size, mycelial growth index (MGI) and final colony diameter (FCD). The universal primers ITS1 and ITS4 amplified a 496 and 450-bp fragment from genomic rDNA of isolates belonging to Pestalotiopsis spp. The comparison of the isolates combining nucleotide sequence obtained by BLAST coupled with morphological criteria (colony appearances and conidia characters) distinguished P. trachicarpicola and Neopestalotiopsis vitis from all other known species in the genera. All isolates were pathogenic to Hungarian grape cultivar 'Poeloeskei Muskotaly' causing necrotic leaf spot and postharvest berry rot. This is the first report of P. trachicarpicola and N. vitis associated with necrotic leaf spot and postharvest rot diseases on grapevine in southern Brazil.
Piva, C.A.G., Bogo, A., Gomes, B., Milcheski, V., Klabunde, G.H.F., Nodari, R.O., da Silva, F.N. and Welter, L.J. (2020). Pestalotiopsis spp. causing grapevine leaf spot and postharvest berry rot in southern Brazil. Acta Hortic. 1280, 209-216
Vitis vinifera, morphology, sequence analysis, pathogenicity