Identifying successful pollinizers of plum cultivars 'Edda' and 'Opal' in Ullensvang, Norway, using microsatellites
In order to identify successful pollinizers of plum cultivars Edda and Opal, 60 mature open pollinated fruit were collected from these two cultivars in autumn 2017. The fruits were harvested in three different orchards located in Ullensvang, the main fruit production area in Norway. After the harvest, kernels were separated from the fruit and used for the extraction of single embryos located within each kernel. At the same time, leaves were collected from the plum cultivars Edda and Opal, as well as from all other plum cultivars present at the location and nearby orchards (Mallard, Jubileum, Reeves, Avalon, Valor, Cacanska Lepotica and Herman). DNA extraction was carried out for the embryos and leaves, and were used for genotyping using seven microsatellite markers. The microsatellite data were used for conducting paternity analyses based on a log likelihood ratio, where the fingerprint information from the leaves were the possible pollen donors available at the location. The results revealed within all examined orchards that the most successful pollinizer of Edda, a self-sterile cultivar, was Opal. The most successful foreign pollinizer of Opal was Mallard. However, more than two thirds of embryos extracted from Opal fruits did not present any alleles not already identified in Opal, which was expected considering that this plum cultivar was known to be self-fertile. European plums are hexaploid making its allele callings and paternity tests rather complicated. However, the approach used in this study gave clear answers regarding the most successful pollinizers.
Meland, M., Frøynes, O., Gasi, F., Kalamujić-Stroil, B. and Lasic, L. (2020). Identifying successful pollinizers of plum cultivars 'Edda' and 'Opal' in Ullensvang, Norway, using microsatellites. Acta Hortic. 1281, 285-288
Prunus domestica L., paternity analyses, sterility, self-fertility, DNA markers