Chromosome doubling of Cichorium intybus L. haploids through protoplast regeneration
Industrial chicory (Cichorium intybus L.) is mainly grown for inulin production.
To enhance production and uniformity, hybrid cultivars are preferred.
Therefore, a controlled pollination system and homozygous parental lines are needed.
The most desirable pollination control system is cytoplasmic male sterility (CMS). A new, naturally induced CMS has been reported by Van der Veken et al. (2018). To regenerate homozygous parental lines of modern industrial chicory cultivars, different haploid inducing techniques have been compared and evaluated.
The only successful method was intergeneric hybridization with Cicerbita alpina (Doré et al., 1996), yielding haploid plants.
Here we show that protoplast regeneration is an efficient tool for chromosome doubling of these haploids.
The presented method can be used for chromosome doubling of slowly developing haploids.
Van der Veken, J., Eeckhaut, T., Baert, J., Maudoux, O., Notté, C., Werbrouck, S. and Van Huylenbroeck, J. (2020). Chromosome doubling of Cichorium intybus L. haploids through protoplast regeneration. Acta Hortic. 1282, 173-178
DOI: 10.17660/ActaHortic.2020.1282.27
https://doi.org/10.17660/ActaHortic.2020.1282.27
DOI: 10.17660/ActaHortic.2020.1282.27
https://doi.org/10.17660/ActaHortic.2020.1282.27
Cichorium intybus L., protoplasts, doubled haploids, chromosome doubling
English
1282_27
173-178
- Division Plant Genetic Resources and Biotechnology
- Division Horticulture for Human Health
- Division Ornamental Plants
- Division Temperate Tree Fruits
- Division Temperate Tree Nuts
- Division Tropical and Subtropical Fruit and Nuts
- Division Vegetables, Roots and Tubers
- Division Vine and Berry Fruits
- Commission Banana
- Working Group Horticultural Biotechnology and Breeding